HPLC復方厚樸顆粒中厚樸酚及和厚樸酚的含量 | HPLC復方厚樸顆粒中厚樸酚及和厚樸酚的含量 |
| 發布時間:2020-05-15 信息來源:admin 發布人:admin 點擊次數:2826 |
建立hplc法測定復方厚樸顆粒中厚樸酚及和厚樸酚的含量。 方法采用diamondsilcmc18色譜柱（6.4 mm×200 mm,5 μm）；流動相為甲醇-0.1%磷酸溶液（78∶22）；流速1 ml/min；柱溫35℃；檢測波長294 nm。結果厚樸酚的線性范圍為0.041 1~0.822 2 μg，r=0.999 9，平均回收率為97.1%,rsd=1.27%；和厚樸酚的線性范圍為0.023 06~0.461 2 μg ,r=1，平均回收率為96.7%，rsd=1.25%。結論該方法結果準確，重現性好，可作為復方厚樸顆粒質量控制的定量方法。 關鍵詞：復方厚樸顆粒； 高效液相色譜法； 厚樸酚； 和厚樸酚 上禾生物 determination of magnolol and honokiol in compound houpu granule by hplc xia hou lin ，sheng yan，zhou xiao mei，wu xi，dong min，tan ling (chengdu university of traditional chinese medicine, 610072 ,china)
厚樸酚1-98% 和厚樸酚1-98% 厚樸總酚 1-98% 上禾生物 原料產地的專業厚樸提取物生產企業--上禾生物 abstract：objectiveto establish an hplc method for the determination of magnolol and honokiol in compound houpu granule.methodsthe samples were analyzed on a diamondsilcmc18 column (6.4 mm×200 mm,5μm). the mobile phase was a mixture of meoh-0.1%h3po4(78:22);the rate of flow was 1ml/min; the column temperature was 35 ℃; the detection wavelength was 294 nm.resultscalibration graph was linear in the range of 0.041 1~0.822 2 μg,r=0.999 9 for magnolol, 0.023 06~0.456 12μg, r=1 for honokiol. the recovery was 97.1%, rsd=1.27% for magnolol; 96.7%,rsd=1.25% for honokiol.conclusionthe method is sensitive and its results are accurate with good reproducibility .it can be used for the quality control of compound houpu granule. key words：compound houpu granule; hplc; magnolol; honokiol
厚樸酚1-98% 和厚樸酚1-98% 厚樸總酚 1-98% 上禾生物 原料產地的專業厚樸提取物生產企業--上禾生物 復方厚樸顆粒由厚樸、藿香、蒼術等藥物組成，具有解表和胃之功效，用于感受風寒，內有食積，寒熱吐瀉等癥。為了控制本品質量，保障用藥安全與有效，本文采用高效液相色譜法測定方中君藥厚樸的主要成分厚樸酚、和厚樸酚的含量。
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